Coding

Part:BBa_K2195000:Design

Designed by: Alex Yenkin   Group: iGEM17_WashU_StLouis   (2017-06-16)
Revision as of 13:10, 16 June 2017 by Registry (Talk | contribs)

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Dsup (Radiation Protection)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 240
    Illegal BamHI site found at 694
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As this protein came from a eukaryotic species, it was not codon optimized for E. Coli. In addition, there were several illegal restriction sites that had to be replaced with different codons.


Source

This comes from the genomic sequence of the Tardigrade Species Ramazzottius varieornatus

References