Part:BBa_K2333001

mf-Lon Protein Degradation Tag A (strong)

This protein degradation tag (pdt) is degraded by the Lon protease from Mycoplasma florum (mf-Lon) LINK TO mf-Lon goes here . This Lon protease system is orthogonal to E. coli endogenous proteases. This tag is part of a series of 6 tags (labeled #3 and #3a-#3e). Of this series of tags, this tag is the strongest, and thus has the highest degradation rate. BBa_K2333001-Bba_K2333006 comprise this tag series. See Collins et al. 2014 "Tunable Protein Degradation in Bacteria" for the original design via mutagenesis, and for their characterization of degradation rates.

Note: Each pdt in the series BBa_K2333001-Bba_K2333006 (pdt#3-pdt#3e) is identical except for mutations on residues 13-15, which is the region of mf-Lon recognition.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]