Device

Part:BBa_M50073:Design

Designed by: Melat Birbo and Charlotte Philp   Group: Stanford BIOE44 - S11   (2017-06-10)
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E. coli pH sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1747
    Illegal AgeI site found at 1859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We engineered pPink to have an ampicillin selection marker with a low copy number of origin of replication (PJ-Amp_Low). We engineered pTurquoise to have a kanamycin resistance with a high copy number of origin of replication (pJ-Kan_High). Since we thought the turquoise fluorescent protein might be more difficult to visualize, we chose to upregulate its production as we could not have a high copy number for both plasmids if inserted in the same organism.


Source

For our promoter in pTurquoise, we used iGEM part BBa_K116001. The turquoise fluorescent protein iGEM part name is BBa_M50029. Following this we used a His tag and a terminator (pA-GH-Bt). For our promoter in pPink, we used iGEM part BBa_K1170000. The RFP iGEM part name is BBa_E1010. Following this we used a FLAG tag and a terminator (pA-GH-Bt).

References