Part:BBa_M50073:Design
E. coli pH sensor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1747
Illegal AgeI site found at 1859 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We engineered pPink to have an ampicillin selection marker with a low copy number of origin of replication (PJ-Amp_Low). We engineered pTurquoise to have a kanamycin resistance with a high copy number of origin of replication (pJ-Kan_High). Since we thought the turquoise fluorescent protein might be more difficult to visualize, we chose to upregulate its production as we could not have a high copy number for both plasmids if inserted in the same organism.
Source
For our promoter in pTurquoise, we used iGEM part BBa_K116001. The turquoise fluorescent protein iGEM part name is BBa_M50029. Following this we used a His tag and a terminator (pA-GH-Bt). For our promoter in pPink, we used iGEM part BBa_K1170000. The RFP iGEM part name is BBa_E1010. Following this we used a FLAG tag and a terminator (pA-GH-Bt).