Reporter

Part:BBa_M50057:Design

Designed by: Jack Akerman and Jon Bartlett   Group: Stanford BIOE44 - S11   (2017-04-26)
Revision as of 00:34, 27 April 2017 by Jakerman (Talk | contribs)


Melanin Pigment with 6xHist Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 100
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 475


Design Notes

The use of Copper and L-tyrosine as precursors for the expression of melanin. We optimized the codons for the E. coli bacteria.


Source

Comes from the Rhizobium etli CFN42 bacteria.

References

http://partsregistry.org/Part:BBa_K274001