Reporter

Part:BBa_M50059:Design

Designed by: Jack Akerman and Jon Bartlett   Group: Stanford BIOE44 - S11   (2017-04-26)
Revision as of 23:48, 26 April 2017 by Registry (Talk | contribs)

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Blackteria: Genetic sequence for the production of melanin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 614


Design Notes

A T-5 promoter is easily inducible and therefore an effective promoter to test the melanin sequence. The strong RBS makes sure that the ribosome binds. The double terminating sequence makes sure transcription terminates.


Source

Rhizobium etli CFN42

References