Regulatory

Part:BBa_M50048

Designed by: Eleanor Glockner, Jack Andraka   Group: Stanford BIOE44 - S11   (2016-12-10)
Revision as of 08:22, 12 December 2016 by Emrglock (Talk | contribs)

This is a riboswitch consisting of an untranslated region at the 5' end (5' UTR), an RNA aptamer that recognizes atrazine, and a ribosome binding site. It is based on BBa_J89000, with the theophylline aptamer exchanged for an atrazine aptamer. In the absence of atrazine, the RNA transcript of this part has excessive base pairing in the 5’ UTR, folding the RNA to block binding at the RBS and thus prevent translation. When atrazine is present, it binds to the aptamer sequence, altering the shape of the transcript to expose the RBS and allow translation of any downstream protein sequences.

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