Regulatory

Part:BBa_M50045:Design

Designed by: Mark Buckup   Group: Stanford BIOE44 - S11   (2016-12-08)
Revision as of 07:25, 12 December 2016 by Mbuckup (Talk | contribs) (→‎Design Notes)

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hCMV Promoter and Kozak


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1052
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 152
    Illegal PstI site found at 826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 888


Design Notes

Mammalian expression is driven by the powerful constitutive promoter and enhancer found in cytomegalovirus, or CMV

Source

TA Robert Chen's plasmid.

References