DNA

Part:BBa_M50043:Design

Designed by: Michael Herschl   Group: Stanford BIOE44 - S11   (2016-12-08)
Revision as of 07:21, 12 December 2016 by Mbuckup (Talk | contribs) (→‎Source)


HIV-1 U3 Region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Recognized by Integrase; these are the terminal 40 base pairs. While the interactions between Integrase and the specific bases of these regions are well studied, and it has been shown that only approximately 20 base pairs are necessary for the protein to bind8,9, we decided to use the terminal 40 base pairs because previous similar experiments have shown that this may increase the efficiency of integration

Source

2006. HIV-1, complete genome. GenBank:AF033819.3.

TA Robert Chen's plasmid.

References