Part:BBa_M50053:Design
FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 77
Design Notes
We engineered E. coli to produce a unique protein composed of two fluorophores - mRuby3 and cometGFP - joined by linkers each to the hinge of a glucose binding protein. This construct will undergo a conformational change upon binding to glucose such that the cometGFP portion in an excited state will transfer energy to the acceptor mRuby3 to be emitted. Quantifying this excitation/emission pair reveals the extent to which FRET and thus glucose binding that has occurred.
Source
Key Parts:
1. mRuby3 Fluorescent Protein - https://parts.igem.org/Part:BBa_M50009
2. 6 Amino Acid Fusion Protein Linker - https://parts.igem.org/Part:BBa_M50036
3. Glucose Binding Protein of Thermus thermophilus - https://parts.igem.org/Part:BBa_M50037