Protein_Domain

Part:BBa_M50037:Design

Designed by: Amanda Urke   Group: Stanford BIOE44 - S11   (2016-12-11)
Revision as of 02:47, 12 December 2016 by Oalimi (Talk | contribs) (→‎Design Notes)


Glucose Binding Protein of Thermus thermophilus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This GBP sequence was optimized for use in E. coli. Also, start and stop codons were removed to facilitate fusion to the other components of our construct.

Source

This GBP comes from the genomic sequence of Thermus thermophilus. The complete bacterial genome was published by Henne et al. in Nature Biotechnology in 2004 (https://www.ncbi.nlm.nih.gov/pubmed/15064768), and the particular GBP was characterized in a 2006 study by Cuneo et al. in the Journal of Molecular Biology (https://www.ncbi.nlm.nih.gov/pubmed/16904687).

References