Composite

Part:BBa_M50039:Design

Designed by: Anika Naidu   Group: Stanford BIOE44 - S11   (2016-11-30)
Revision as of 23:50, 11 December 2016 by Abbiemcnulty (Talk | contribs) (→‎Source)


E. coli positive feedback cusR producer (short promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 53
    Illegal AgeI site found at 243
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed a genetic construct where the first half consisted of the cusR inducible promoter, a strong ribosome binding site (RBS) listed in the Parts Registry (BBa_B0035) and thecusR gene sourced from NCBI.


Source

References