Protein_Domain

Part:BBa_M50036

Designed by: Amanda Urke   Group: Stanford BIOE44 - S11   (2016-12-11)
Revision as of 22:54, 11 December 2016 by Registry (Talk | contribs)

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6 Amino Acid Fusion Protein Linker

This sequence encodes for six amino acids that constitute the fusion protein linker for our Stanford BioE44 DNA construct. It is optimized for use in E. coli, and is designed to maintain linearity of the sequence and prevent protein folding. Our design relied on proper orientation of two GBP domains linked on each end to a flurophore. The linkers were included to ensure that glucose-binding caused the conformational change characterized with FRET instead of inherently folded protein structure, the linkers were included.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None