Part:BBa_M50050:Experience

General Information

Applications of BBa_M50050

• Standardized growth control for bacteria species that have pathways for sensing and responding to Autoinducer-2.

Stanford Location

Plasmid Name: LuxS Synthase Plasmid

DNA 2.0 Gene #: pD441-CH

Organism: E. coli

Device Type: Actuator

Glycerol Stock Barcode: 0133027189

Box Label: BIOE44 F16

Data

testing

Figure

Data Analysis and Conclusions

All figures show comparisons between the LuxS transfected bacteria, a wild-type control strain, and a similar IPTG-inducible E. coli strain with a T5 promoter (Pink).

Western Blot

Based on our analysis of our Western blot, we concluded that we were successful in transfecting our E. coli strain with the correct plasmid and gene sequence. For BBa_M50050, we looked at well sections A and B (BBa_M50050 transfected E. coli at 0 μM and 1000 μM in-solution concentration respectively) compared to the wild-type controls (C and F). What we see is that there are bands of proteins located at the 40 kilodaltons (the anticipated length) in sections A and B, but not in the control sections C and F.

Cell Density Tests

Based on the data collected from our overnight cell density tests, we can conclude that the over-expression of LuxS increases cell growth during the exponential growth phase, but does not result in any vastly different final cell density concentrations.

Experimental Procedures

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