Generator

Part:BBa_M50017:Design

Designed by: Taylor Merkel, Chloe Thai, Julia Schulz   Group: Stanford BIOE44 - S11   (2016-10-27)
Revision as of 00:47, 11 December 2016 by Tmerkel (Talk | contribs) (→‎References)


d-Limonene Synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1531
    Illegal SapI.rc site found at 253
    Illegal SapI.rc site found at 1506


Design Notes

Gene for d-limonene synthase, optimized for usage in E. coli based on G/C and codon abundance

Source

Derived from BBa_K1660003, based on sequence for d-limonene synthase in Citrus unshiu

References

https://www.ncbi.nlm.nih.gov/nuccore/AB110636.1, BBa_K1660003