Regulatory
Part:BBa_K1955003
Designed by: Justine Hsu Group: iGEM16_CGU_Taiwan (2016-10-14)
pSB1C3-5'HYG
We replaced p36 and nagt genes with a cloning site for an exogenous gene and with hyg as a selection marker, respectively. This dual-function biobrick enables stable expression of foreign proteins by drug selection in Leishmania.
(1) The basic part checked by PCR:
We used pSB1C3-5’HYG, pSB1C3-3’UTR, pSB1C3-HA, pSB1C3-OVA as template, to check the length of the inserts. The PCR reaction was performed with Taq polymerase, and screened in 0.8％ agarose gel by electrophoresis.
Sequence and Features
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Categories
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