Regulatory

Part:BBa_J100311:Design

Designed by: Monica Prudencio   Group: Campbell M Lab   (2016-12-02)
Revision as of 16:39, 2 December 2016 by Registry (Talk | contribs)

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Scrambled TetR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We were cautious to scramble only those bases involved in TetR binding, while keeping other bases the same in order to maintain as best we can the spacing and structure of the original promoter.


Source

The original sequence is the TetR repressible promoter, R0040. Binding regions were identified from Orth P, et al., "Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system." DOI: 10.1038/73324


References