Coding

Part:BBa_K2110200

Designed by: Haoran Ding   Group: iGEM16_Tianjin   (2016-10-13)
Revision as of 13:21, 16 November 2016 by Dhr9767 (Talk | contribs)

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PETase expression system in Saccharomyces cerevisiae

Saccharomyces cerevisiae is a widely used chassis organism in synthetic biology research. We use it as the chassis organism to express the PETase gene and assay the enzyme activity. This composite part consists of the enhanced promoter PGK1 (BBa_K2110012), α signal peptide (BBa_K2110017), PETase gene (or its various mutants, BBa_K2110800, BBa_K2110100-BBa_K2110109), and CYC1 terminator (BBa_K2110000). The expression data can be accessed respectively in the part information page of PETase gene and its mutants. (BBa_K2110800, BBa_K2110100-BBa_K2110109).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1141
    Illegal BglII site found at 1351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisSaccharomyces cerevisiae