Composite

Part:BBa_J100204

Designed by: Monica Prudencio   Group: Campbell M Lab   (2014-12-05)
Revision as of 21:33, 5 November 2016 by Macampbell (Talk | contribs)

actClone Red

We designed actClone Red to test the power of activators. The two BsaI sites allow the reverse primer and transcriptional terminator to be removed and a DNA segment that binds to an activator to be ligated in their place, causing translation of RFP. This can be used in synthetic biology laboratories or for undergraduate introductory biology lab modules.

The OmpR-activated ompC promoter (R0082) is only present in part. The new insert will contain the 5' end of this promoter, allowing transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 1739
    Illegal AgeI site found at 1612
    Illegal AgeI site found at 1724
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 928
    Illegal BsaI.rc site found at 817
    Illegal SapI site found at 708


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