Generator

Part:BBa_K2012018

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-07)
Revision as of 14:39, 1 November 2016 by HZAU BGMa (Talk | contribs)


A medium CheZ generator This generator is altered from BBa_K819010 created by Peking University.Its previous promotor , J23113,is replaced by a medium promotor J23106. This generator is constructed to detect the relationship between different promoters' strength with their swarming ability. A series of Biobricks were constructed as BBa_K2012017 [https://parts.igem.org/Part:BBa_K2012017], BBa_K2012020 [https://parts.igem.org/Part:BBa_K2012020]


Figure 1. The swarming plate of different E.coli K12 cheZ lacking strain, CL1, transformed with our constructed plamids.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None