Reporter
GG95

Part:BBa_K2145000:Experience

Designed by: Laura Xinyu Chen, Melody Wu, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-13)
Revision as of 23:50, 30 October 2016 by Sclamons (Talk | contribs)

Splates95102.png

Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG#### above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

Alverno ca a95.png Alverno ca r 95.png Alverno ca g 95.png

Shown above are OD600 measurements, RFP fluorescence, and GFP fluorescence, respectively, for three clones of this part. Again, we emphasize that only RFP OR GFP are expressed, but never both. We believe this may be due to supercoiling -- when one gene expresses, it creates supercoils that shut down the other gene.

Sequencing on this plasmid was low-quality and inconclusive.


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