Reporter
GG96

Part:BBa_K2145001

Designed by: Melody Wu, Laura Xinyu Chen, Katie Maruna   Group: iGEM16_Alverno_CA   (2016-10-14)
Revision as of 23:12, 30 October 2016 by Mwu (Talk | contribs)


GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) along with a "spacer" part and pSB1C3 backbone assembled to make the final part. It can be used to express both GFP and RFP proteins. Direction of GFP: Forward, toward the spacer; Direction of RFP: Forward, away from the spacer

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2270
    Illegal AgeI site found at 2382
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None