Composite

Part:BBa_K2139016

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)
Revision as of 20:45, 30 October 2016 by Dchristy (Talk | contribs)

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CenA with Terminator


Cellulomonas fimi uses 3 endoglucanases (including CenA, accession M15823) and an exoglucanase in the degradation of cellulose into cellobiose, before using beta-glucosidase to catalyse the conversion of cellobiose to D-glucose. This part encodes cenA and a double terminator. This part was codon optimized for the expression in Caulobacter crescentus and synthesized by IDT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 723
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None