Part:BBa_K1961001

RecF protein-SOS mediator protein

The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. When DNA is damaged and forms single-stranded DNA, Rec A binds single-stranded DNA (ssDNA). The binding of RecA onto ssDNA forms a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response). Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA.

Background

RecA is a protein in nearly all bacteria and all organisms. Following a break on a single-stranded DNA (ssDNA), the exonuclease RecJ cleaves single-stranded DNA. The single-strand DNA-binding protein (SSB) binds to the daughter strand gap, preserving the integrity of the DNA. Recombinase mediator proteins RecF, RecO, and RecR act together to promote loading of RecA onto single stranded DNA. Rec A binds single-stranded DNA (ssDNA), forming a nucleoprotein filament that promotes LexA cleavage (thereby inducing the SOS response).

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This figure is obtained from the reference below.

Refernce
Naofumi Handa, Katsumi Morimatsu, Susan T. Lovett, Stephen C. Kowalczykowski. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. GENES & DEVELOPMENT (2009). 23(10):1234–1245 Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 229
  Illegal SapI.rc site found at 395