Composite

Part:BBa_K2139006

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)
Revision as of 20:00, 30 October 2016 by Dchristy (Talk | contribs)


Ptac RBS Gluc1C

Ba_K2139006 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C along with ptac and rbs. Gluc1C is a monomeric enzyme capable of catalyzing the degredation of cellobiose down to two glucose. It can be used in conjunction with an exo-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 351
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1225


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Categories
Parameters
None