Composite

Part:BBa_K2139005

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)
Revision as of 19:46, 30 October 2016 by Dchristy (Talk | contribs)


Ptac RBS Endo5a

Endo5a Expression Cassette without Terminator BBa_K2139005 encompass the coding region for an endo-beta-1,4-glucanase known as Endo5A along with Ptac and an RBS. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exo-beta-1,4-glucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX (homolog). This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1062
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 493
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 390


[edit]
Categories
Parameters
None