Coding
ecf16
Part:BBa_K2070001:Design
Designed by: iGEM UT-Tokyo 2016 Group: iGEM16_UT-Tokyo (2016-10-09)
Revision as of 16:01, 30 October 2016 by RinaSakata (Talk | contribs)
ecf16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 177
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 372
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part sequence was designed with reference to amnio acid sequence of the ECF sigma provided by Rhodius V.A et al (2014). Specific sequences were selected so that no illegal cut would be introduced and so that parts could be synthesised by IDT gene fragments.
Source
Pseudomonas entomophila L48
References
Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58