Composite

Part:BBa_K2180013

Designed by: Wilhelm Vaysse-Zinkhöfer   Group: iGEM16_UPMC-Paris   (2016-10-14)
Revision as of 06:28, 30 October 2016 by Wilhelm (Talk | contribs)

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Promoter + Zinc Operator - CzrA Repressor Binding Site + Blue Pigment + Terminator

This biobrick allow the production of the blue pigment in the presence of zinc.
This is the assembly of our parts BBa_K2180001, BBa_K2180006 and BBa_K2180008

T--UPMC-Paris--Seq2b.png
The insert can also be confirmed by digestion using a unique restriction site inside the biobrick and checked by electrophoresis migration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1039
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None