Translational_Unit
Cld(-SP)

Part:BBa_K2179003

Designed by: michael ellison   Group: iGEM16_UrbanTundra_Edmonton   (2016-10-21)
Revision as of 23:43, 29 October 2016 by Ethanagena (Talk | contribs)

Translational Unit for Chlorite Dismutase (Cld)

Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 807
    Illegal BsaI.rc site found at 6

[edit]
Categories
Parameters
None