Coding

Part:BBa_K2074034

Designed by: Ruicheng Dai   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 17:42, 29 October 2016 by Wzj2016 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cry11Aa(Codon optimization)+2A+EGFP(Codon optimization) 800px-T--FAFU-CHINA--partsclones.png

We use 2A system to link cry11Aa and EGFP then transferred the gene into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .

'
T--FAFU-CHINA--Result3.png
K2074034.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
    Illegal NgoMIV site found at 2740
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal SapI.rc site found at 1440


[edit]
Categories
Parameters
None