Part:BBa_K2165002:Design

Violacein C gene with CUP1 promoter + ADH1 terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 370
  

Design Notes

This biobrick utilizes the University of Washtion's Cup1 promoter. It has had one base pair mutated to remove an illegal XbaI cut site. VioC was codon optimized in hopes of improving the expression. The biobrick ends with the ADH1 terminator <a href="https://parts.igem.org/Part:BBa_K801012" target="_blank">BBa_K801012</a> for yeast that was submitted by the TU Munich team in 2012. For more information about each part, view there respective pages.

Source

VioC is derived from the violacein pathway in ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The original VioC sequence was aquired from the Duber laboratory at the University of California Berkley and was codon optimized through IDT's codon optimizer web application. The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in S. cerevisiae. The ADH1 terminator is also from the genomic DNA of S. cerevisiae.

Characterization

Figure 1: Constituatively active VioABCDE yeast in synthetic media, along with a positive control (regular yeast) and a negative control (no yeast)