Generator

Part:BBa_K1915001

Designed by: Holly Bowman   Group: iGEM16_Georgia_State   (2016-10-05)
Revision as of 15:56, 29 October 2016 by Jrichardson24 (Talk | contribs)

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Inducible Lac promoter+LTNF10

This is the generator part for Bba_K1915000. In this part an IPTG inducible lac promoter is included immediately after the prefix. The RBS J61101 proceeds LTNF10 coding sequence. This is followed by a Human Rhino Virus protease site and then Myc and 6xHIS tags on the tail end.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 165
    Illegal BamHI site found at 99
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None