Composite

Part:BBa_K1952011

Designed by: Jessica Zhao   Group: iGEM16_Kingsborough_NY   (2016-10-19)
Revision as of 15:54, 29 October 2016 by Djcamenares (Talk | contribs)

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Hydrazine Synthase subunit beta (Kust-2860) with LacZ reporter

Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter, followed by LacZ alpha subunit reporter gene.

Used to verify proper expression of Hydrazine Synthase beta subunit in E. coli (demonstrate expression by blue color of bacteria in the presence of IPTG and X-gal.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 966
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None