Composite

Part:BBa_K1952010

Designed by: Jessica Zhao   Group: iGEM16_Kingsborough_NY   (2016-10-19)
Revision as of 15:48, 29 October 2016 by Djcamenares (Talk | contribs)


Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter

Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).

Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal XhoI site found at 1474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
    Illegal AgeI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None