Composite

Part:BBa_K1952010:Design

Designed by: Jessica Zhao   Group: iGEM16_Kingsborough_NY   (2016-10-19)
Revision as of 15:46, 29 October 2016 by Djcamenares (Talk | contribs)

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Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 445
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal XhoI site found at 1474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 568
    Illegal AgeI site found at 1461
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Utilized DNA from construction of K1952000 together with other spacer DNA and the coding region of LacZ alpha (for Blue-White screening) in Gibson assembly. Interchangable with parts for K1952009 assembly.


Source

DNA synthesized by Integrated DNA technologies.

References

Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]