Protein_Domain
Kust2860

Part:BBa_K1952001:Design

Designed by: Alex Dean   Group: iGEM16_Kingsborough_NY   (2016-10-14)
Revision as of 15:29, 29 October 2016 by Djcamenares (Talk | contribs)


Hydrazine Synthase beta subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1032
    Illegal BamHI site found at 501
    Illegal BamHI site found at 702
    Illegal BamHI site found at 825
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 178


Design Notes

This construct contains the coding sequence only, reverse translated from K. stuttgartiensis protein sequence, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.


Source

K. stuttgartiensis protein sequence; GenBank CAJ73612.1 [1]

References

Kartal, B., et al (2011) Molecular Mechanism of Anaerobic Ammonium Oxidation. Nature Vol 479 [doi:10.1038/nature10453]