Protein_Domain

Part:BBa_K1952000

Designed by: Alex Dean   Group: iGEM16_Kingsborough_NY   (2016-10-14)
Revision as of 15:21, 29 October 2016 by Djcamenares (Talk | contribs)


Hydrazine Synthase gamma subunit

The gamma subunit of Hydrazine Synthase from Kuenenia stuttgartiensis. This construct contains the coding sequence only, optimized for expression in E. coli, with subsequent removal of internal restriction sites and repetitive sequences.

This enzyme is a key step in the anaerobic ammonia oxidation pathway, which converts ammonia (NH4) and nitric oxide (NO) into nitrogen gas (N2). The Hydrazine Synthase enzyme takes the two starting reactants and produces the intermediate hydrazine (N2H4).

For more information see Kartal, B., Keltjens, J., (2016) Anammox Biochemistry: A Tale of Two Heme c proteins. Trends in Biochemical Sciences. [http://dx.doi.org/10.1016/j.tibs.2016.08.015]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 885
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None