Composite

Part:BBa_K2066117:Experience

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 05:59, 29 October 2016 by Lkolla (Talk | contribs) (→‎Applications of BBa_K2066117)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

This part is sequence confirmed

Applications of BBa_K2066117

This synthetic enhancer part includes a 2x TetO binding cassette in the spacer region between the enhancer and sigma 54 promoter, and depending on the steady state of TetR, the rigidity of DNA changes and thus affects the thermodynamic capability of looping and activation of transcription.

Synthetic enhancer sequences were derived from Amit et. al. 2011 and UNS sequences from Torella et. al. 2012.

For more information on the mechanism of the synthetic enhancer circuit, refer to our page: http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer.

User Reviews

UNIQ12f25dbe5bc2a06d-partinfo-00000000-QINU UNIQ12f25dbe5bc2a06d-partinfo-00000001-QINU