Part:BBa_K1915003:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1915003

Figure 1. Gel of Inducible Lac Promoter+LTNF-15. The above is a gel of the colony PCR of the inducible lac promoter+LTNF-15.

Inducible lac promoter + LTNF-15/i> Colony PCR of transformed inducible lac promoter + LTNF15 in PSB1C3. Lanes 1,2,3,4,5,7,9,10 gave us bands around 500 basepairs which is what was expected for the PCR reaction using our VF2 and VR primers. See the figure 1 above.


Figure 2. Gel of Inducible Lac Promoter+LTNF-15. The above is a gel of the inducible lac promoter+LTN-15 digested with BamHI.

LTNF has a BamHI restriction site that we utilized to help diagnose if the inducible lac promoter + LTNF15 was in the pSB1C3 vector. The first lane contains the plasmid lacking the BamHI restriction site. Each restriction digest was treated the same. Lanes 2,3,4, and 5 have the inducible lac promoter + LTNF15 cut with BamH1. Bands appear around the expected 2289 bp.See the figure 2.

Figure 3. Gel of Inducible Lac Promoter+LTN-15. The above is a gel of the inducible lac promoter+LTN-15 digested with EcoRI and PstI.

A restriction digest of the inducible lac promoter + LTNF15 was done using EcoRI and Pst1. The plasmid pSB1C3 served as a control because we knew that the bands produced would be larger in size than the bands produced from our digest of BBa_ K1915003. In lane 1 the plasmid pSB1C3 with RFP was digested with EcoRI and in lane 2 the plasmid pSB1C3 with RFP was digested with EcoRI and PstI. Lanes 4,6,8, and 10 show the inducible lac promoter + LTNF15 around the expected size of 260 bp.See the figure 3 above.

User Reviews

UNIQc272fcafd002f707-partinfo-00000000-QINU UNIQc272fcafd002f707-partinfo-00000001-QINU