Part:BBa_K1893002
Rhl receiver (RhlR+pRhl)
The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl.
The Rhl receiver device consists of a constitutively expressed RhlR transcription regulator (BBa_C0171) upstream of a cognate pRhl promoter (BBa_R0071). RhlR is activated in the presence of a C4-AHL signal and binds to pRhl to initiate transcription of a downstream coding sequence.
Usage and biology
The Rhl receiver is based on the Rhl quorum-sensing system from Pseudomonas aeruginosa. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression.
In this case, the constitutively expressed RhlR transcriptional regulator (BBa_C0171) is activated by the binding of C4-AHL, an AHL quorum signal. The activated RhlR regulator binds to the RhlR-inducible promoter, pRhl, to initiate transcription of a downstream gene.
Characterisation
We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here (BBa_K1893003).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 776
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