Measurement

Part:BBa_K2066037

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-08)
Revision as of 03:13, 29 October 2016 by Ethan801 (Talk | contribs)


pACT-Tet on UNS Standard

This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper on the UNS standard. This part consists of the endogenous nitrogen regulatory promoter, the NRII mutant BBa_K20660112 and tetR. This is meant for use in Tet based synthetic enhancer systems, such as the [http://2016.igem.org/Team:William_and_Mary/Synthetic_Enhancer William and Mary 2016]

Note this part does not have a terminator, as the plasmid it was derived from used the terminator of the origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None