Part:BBa_K2165000

Violacein C gene codon-optimized for S. Cerevisiae

VioC is the final (fourth, or fifth if VioD also present) enzyme in the violacein pathway when expressed. VioC, alongside VioA, VioB, and VioE, processes its substrate protodeoxyviolaceinic acid into deoxyviolaceinic acid. Deoxyviolaceinic acid then decays, producing a pink-color pigment. When VioD is also expressed, protoviolaceinic is instead used as VioC's substrate, producing an intermediate which subsequently degrades into a dark, purple pigment called volacein.

VioC was previously available as a standalone part as BBa_K539513, produced by [http://2011.igem.org/Team:NCTU_Formosa NCTU Formosa's 2011 iGEM team]; however, BBa_K539513 is not codon optimized for yeast. The part developed by the University of Washington's iGEM team have been codon optimized for yeast in hopes to improve the efficiency of its translation. The CDS contained in this BioBrick was designed by running the VioC gene sent to the University of Washington by the [http://dueberlab.berkeley.edu/ Dueber Laboratory] at University of California-Berkley through IDT's Codon Optimization Tool for S. cerevisiae.

Usage and Biology

It should be stressed that without the other main components of the Violacein pathway (VioA, VioB, and VioE, see part BBa_K726015 as an example), the substrate for VioC and VioD will not be produced and no pigment will be visible.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 101