Composite

Part:BBa_K1877001:Design

Designed by: Ruijie Tang   Group: iGEM16_Gaston_Day   (2016-09-19)
Revision as of 00:14, 29 October 2016 by RuijieTang (Talk | contribs) (→‎Design Notes)


pBAD + RBS + GFP + Single Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


Design Notes

This is the testing construct for arabinose inducible mechanism and also serves as the construct, which helps us to determines the optimal concentration of arabinose of inducing the pBAD promoter. In response to arabinose concentration increase, the pBAD promoter should start the production of GFP. So, in a lab environment, like plates, the pBAD promoter should be continuously producing GFP. We test the production of GFP with fluorescences per OD600 through an spectrophotometer.

Source

All separate genes come from the registry.

References