Composite

Part:BBa_K1877011:Design

Designed by: Ruijie Tang   Group: iGEM16_Gaston_Day   (2016-09-21)
Revision as of 00:12, 29 October 2016 by RuijieTang (Talk | contribs) (→‎Design Notes)


Tetr Promoter+RBS+GFP+Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

The construct is designed to test along with BBa_K1877001. This is the testing construct for the repressible mechanism of 2016 Gaston Day School iGEM team's project, killswitch. If this construct is placed by itself in medium containing arabinose, it should be producing GFP nonstop, and we tested it with fluorescences per OD 600. After testing its individual function, we, then, ligate BBa_K1877031 along with this composite part. Then, we put them into different concentrations of arabinose. With the Tetr repressor repressing the Tetr repressible promoter, the GFP concentration should decrease in response to an increase of arabinose concentration.

Source

iDT and the registry

References