Regulatory
pBAD

Part:BBa_K1893015:Design

Designed by: Henry Lloyd-Laney, Stefan Grossfurthner   Group: iGEM16_Imperial_College   (2016-10-14)
Revision as of 20:00, 28 October 2016 by Cmoller (Talk | contribs) (→‎Design Notes)

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Arabinose inducible promoter (pBAD)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1098


Design Notes

We added a reverse terminator to the original pBAD promoter system in order to terminate translation of the araC coding sequence in plasmid backbones that lack an upstream terminator, such as pSB1A2.

Source

This part was created by ligating the araC and pBAD inducible promoter construct in the distribution kit (BBa_K1321333) with the BBa_B0025 reverse terminator

References