Part:BBa_K1893015
Arabinose inducible promoter (pBAD)
This composite part is composed of a pBAD arabinose inducible promoter system developed by the 2014 Imperial team (BBa_K1321333) and an upstream reverse terminator (BBa_B0025) to terminate transcription of an antisense encoded araC transcription factor in the pBAD system. This part enables arabinose inducible expression of a downstream coding sequence, and can be switched off by the addition of D-glucose.
Usage and Biology
When arabinose is absent, araC forms a homodimer and binds to operator sites upstream of the pBAD promoter. This generates an inhibitory DNA loop that blocks RNA polymerase binding and prevents transcription. In the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of the coding sequence downstream of the pBAD promoter. The promoter can then be switched off via catabolite repression by adding D-glucose to the system.
We have characterised the activation range of this part using GFP as a reporter. The results of our these characterisation experiments can be found here (BBa_K1893017).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1098
| None |