Project

Part:BBa_K1996004

Designed by: Claudia Moratti   Group: iGEM16_Sydney_Australia   (2016-10-14)
Revision as of 17:13, 28 October 2016 by Claudia Moratti (Talk | contribs)


Purple chromoprotein

Purple chromoprotein

Usage and Biology

Error-prone PCR was conducted to produce alternative version of the blue chromoprotein amilCP submitted by Uppsala 2011 (BBa_K592009). One of these versions is this purple chromoprotein.

T--Sydney_Australia--amilCPmutants.png


Figure 1. Purple, pink and green mutants compared to the wild type.
Protein was extracted from 50mL of culture and resuspended in 0.5mL TE buffer.

T--Sydney_Australia--amilCPspectrum3.png
Figure 2. Spectrum scan of the extracted protein from the different coloured amilCP mutants. Protein concentration is equal between all samples.

T--Sydney_Australia--amilCPspectrum4.png
Figure 3. Spectrum scan of the extracted protein from the different coloured amilCP mutants, with the spectrums all adjusted to the same levels to show peaks.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None