Coding

Part:BBa_K2043009:Design

Designed by: Mislav Acman   Group: iGEM16_Paris_Bettencourt   (2016-10-13)
Revision as of 03:37, 28 October 2016 by Shruthi31 (Talk | contribs)

_ bpuI-FBD10 laccase codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

bpuI-FBD10 laccase sequence was checked and corrected for potential BpiI, BsaI and BsmBI recognition sites. These sites cannot be present in the coding sequence, because the BpiI, and BsaI enzymes are widely used in phytobrick cloning (http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks). This BioBrick is the bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 10 (FBD10) on the 5'-end of the sequence using Golden Gate assembly method.
FBD 10 was a positive control obtained from literature. This 7 amino acid sequence was obtained by the phage display technique. They screened for small peptide ligands that selectively bind to the Cellulose nano whiskers.


Source

Bacilus pumillus

References

"Screening for cellulose nanowhiskers binding peptides by phage display." Guo, Jing, et al. 2010 Pittsburgh, Pennsylvania, June 20-June 23, 2010. American Society of Agricultural and Biological Engineers, 2010.