Intermediate

Part:BBa_K1976051

Designed by: Alana Gouveia, Franziska Hameister, Jonas Sindlinger, Maik Schork   Group: iGEM16_TU_Darmstadt   (2016-10-14)
Revision as of 02:58, 28 October 2016 by Franny3112 (Talk | contribs)


Trypsin Fragment of Colicin E2 with B0034 as a RBS

Fragment of Colicin E2 as if treated with trypsin with B0034 as a ribosomal binding site.

The DNase trypsin fragment (part 49) can be obtained by trypsin digestion of colicin E2. In this part the gene is fusioned to the strong ribosomal binding site B0034.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None