Regulatory

Part:BBa_K2123108

Designed by: Maria Clara Tavares Astolfi   Group: iGEM16_UFAM-UEA_Brazil   (2016-10-13)
Revision as of 20:41, 27 October 2016 by Mctastolfi (Talk | contribs)


Improved Mer Operon Device with novel regulated promoter

Overview

In 2014, our team constructed a synthetic genetic circuit which reached 74% of mercury bioremediation. Now, this composite part was developed to improve the mer operon expression, related to mercury metabolism, aiming to increase Hg. In order to do so, we designed 8 new promoters regulated by mer operon system. This composite part is the final device with the best of the regulated promoters You can see the promoter characterization here: http://2016.igem.org/Team:UFAM-UEA_Brazil/Project/NewPromoters


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 24
    Illegal NheI site found at 28
    Illegal NheI site found at 32
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None