Part:BBa_K1893031

STAR target (pAD1.S5) For designing a small transcriptional activating RNA through an anti-terminator mechanism, Chappell et. al. investigated transcriptional terminators from different plasmids and of different lengths and sequences. Of these, the sense pAD1 plasmid attenuator sequence no. 5 (pAD1.S5) and its associated STAR-antisense sequence no. 5 (pAD1.A5) pair gave the highest (94-fold) activation of the reporter gene, SFGFP [Chappell et al., 2015]. Thus we used the pAD1.S5 sequence as our STAR target-encoding DNA and the pAD1.A5 sequence as the STAR-antisense-encoding DNA. When transcribed, this part produces the sense RNA which is also complementary to the STAR-antisense RNA. In the absence of STAR, it forms a hairpin structure and and prevent transcription of the downstream coding sequence.

Secondary Structure

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Measurement

• [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]